Journal: Alzheimer's Research & Therapy

Article Title: p75 neurotrophin receptor shapes the dynamics of adult hippocampal neurogenesis in Alzheimer’s disease

doi: 10.1186/s13195-026-01989-7

Figure Lengend Snippet: The effects of p75NTR deficiency on the proliferation, differentiation and maturation of NSCs. A Coronal sections, of the hippocampal DG from 2 months old WT and p75NTR ko mice injected with BrdU for 5 days. Sections were co-immunostained for BrdU (red) and Sox2 (green). Scale bar, 100 μm. B Quantification of both BrdU + and Sox2 + cells in injected mice (2mo -- n = 8 for each genotype; 4mo & 6mo -- n = 5 for each genotype). Data are presented as mean ± SEM. 2way ANOVA **** p < 0,0001, * p < 0,0,5 ns, no significant. C Coronal sections, of the hippocampal DG from 2 months old WT & p75NTR ko mice. Images depict Dcx (red) immunostained immature neurons. Scale bar, 100 μm. D Quantification of Dcx + cells in p75NTR ko & WT mice (2mo & 4mo -- n = 5 for each genotype, 6mo -- n = 3 for each genotype). Data are presented as mean ± SEM. 2way ANOVA** p < 0,005, ns, no significant. E Coronal sections, of the hippocampal DG from 2 months old WT mouse. Images depict BrdU (red) & NeuN (green) immunostained mature neurons. Scale bar, 100 μm. F Quantification of both positive cells in 2 months old p75NTR ko & WT mice ( n = 3 p75NTR ko, n = 3 WT). Data are presented as mean ± SEM. * p < 0.05 (Student’s t-test unpaired). G Coronal sections of the hippocampal DG from 2 months old WT mouse. Images depict caspase3 + cells (red), Hoechst (blue). Scale bar, 100 μm. H Quantification of caspase3 + cells in 2 months old WT & p75NTR ko mice ( n = 3 p75NTR ko, n = 3 WT). Data are presented as mean ± SEM. *** p < 0.001 (Student’s t-test unpaired). BrdU, 5-bromo-2′-deoxyuridine; Sox2, SRY-Box Transcription Factor 2; Dcx, doublecortin; NeuN, Neuronal Nuclear marker

Article Snippet: Wild-type (WT), p75NTR ko, p75 fl/fl NestinCre, 5xFAD and 5xFAD/p75NTR ko male and female mice of different ages (2, 4 and 6-months old) were used. p75NTR ko mice (Ngfrtm1Jae ExonIII) and 5xFAD heterozygous mice (#002213 and #034848-JAX, respectively) were maintained on a C57BL/6 background.

Techniques: Injection, Marker

Journal: Alzheimer's Research & Therapy

Article Title: p75 neurotrophin receptor shapes the dynamics of adult hippocampal neurogenesis in Alzheimer’s disease

doi: 10.1186/s13195-026-01989-7

Figure Lengend Snippet: Functional analysis of differential gene expression driven by the p75NTR ko genotype. A Volcano plot showing the number of Up (9) and Down (33) regulated genes (p adj < 0.05), pointing out the differential expression of Ngfr. Differential expression was determined using a Generalized Linear Model (EdgeR) identifying the main effect of the p75NTR ko genotype (p75NTR ko and 5xFAD/p75NTR ko mice, relative to WT and 5xFAD mice), adjusting for disease status and gender. B Bubble plot showing selected gene ontology (GO) and pathways significantly enriched by the complete list of significant DEGs (both upregulated and downregulated genes) identified in ( A ). Analysis was performed using Metascape (https://metascape.org). The x axis represents the gene ratio. Size of the bubble represents the numbers of DEGs found in each GO list. Coloration from yellow to black represents the – Log10_P value and depicts low (yellow) to high (black) enrichment scores (p adj < 0.05). C Heatmap showing the expression profile of all differentially expressed genes (p adj < 0.05) between samples with (5xFAD/p75NTR ko - green, p75NTR ko - blue) and without (5xFAD - yellow, WT - purple) the p75NTR receptor knocked out. Scaling was performed across genes using z-scores, to highlight relative expression differences. Fold changes are represented using the log₂ fold change (log₂FC) scale to reflect differential expression between conditions

Article Snippet: Wild-type (WT), p75NTR ko, p75 fl/fl NestinCre, 5xFAD and 5xFAD/p75NTR ko male and female mice of different ages (2, 4 and 6-months old) were used. p75NTR ko mice (Ngfrtm1Jae ExonIII) and 5xFAD heterozygous mice (#002213 and #034848-JAX, respectively) were maintained on a C57BL/6 background.

Techniques: Functional Assay, Gene Expression, Quantitative Proteomics, Expressing

Journal: Alzheimer's Research & Therapy

Article Title: p75 neurotrophin receptor shapes the dynamics of adult hippocampal neurogenesis in Alzheimer’s disease

doi: 10.1186/s13195-026-01989-7

Figure Lengend Snippet: p75NTR deficiency in p75 floxed/floxed NestinCre mice is not affecting the proliferation and differentiation of NSCs. A Coronal sections of the hippocampal DG from 2 months old WT and p75 fl/fl NestinCre mice injected with BrdU for 5 days. Sections were co-immunostained for BrdU (red) and Sox2 (green). Scale bar, 100 μm ( B ) Coronal sections of the hippocampal DG from 2 months old WT & p75 fl/fl NestinCre mice. Images depict Dcx (red) immunostained immature neurons. Scale bar, 100 μm. C Quantification of BrdU + and Sox2 + cells in injected mice ( n = 6 for each genotype). Data are presented as mean ± SEM. p**** < 0,0001, ns, no significant (one way ANOVA). D Quantification of Dcx + cells in 2 months old WT & and p75 fl/fl NestinCre mice ( n = 5 for each genotype). Data are presented as mean ± SEM. * p < 0,05, ns, no significant (one way ANOVA). BrdU, 5-bromo-2′-deoxyuridine; Sox2, SRY-Box Transcription Factor 2; Dcx, doublecortin

Article Snippet: Wild-type (WT), p75NTR ko, p75 fl/fl NestinCre, 5xFAD and 5xFAD/p75NTR ko male and female mice of different ages (2, 4 and 6-months old) were used. p75NTR ko mice (Ngfrtm1Jae ExonIII) and 5xFAD heterozygous mice (#002213 and #034848-JAX, respectively) were maintained on a C57BL/6 background.

Techniques: Injection

Journal: Alzheimer's Research & Therapy

Article Title: p75 neurotrophin receptor shapes the dynamics of adult hippocampal neurogenesis in Alzheimer’s disease

doi: 10.1186/s13195-026-01989-7

Figure Lengend Snippet: The effects of p75NTR deficiency on the proliferation and differentiation of NSCs, under Alzheimer’s Disease. A Coronal sections, of the hippocampal DG from 2 months old 5xFAD/p75NTR ko mouse injected with BrdU for 5 days. Sections were co-immunostained for BrdU (red) and Sox2 (green). Scale bar, 100 μm ( B ) Quantification of BrdU + and Sox2 + cells in injected mice. (2mo & 4mo - n = 5 for each genotype). Data are presented as mean ± SEM. 2way ANOVA**** p < 0,0001, *** p < 0,001, p**< 0,005. C Coronal sections, of the hippocampal DG from 2 months old WT, p75NTR ko, 5xFAD and 5xFAD/p75NTR ko mice. Image depicts Dcx (red) immunostained immature neurons. Scale bar, 100 μm. D Quantification of Dcx + cells in WT, p75NTR ko, 5xFAD, and 5xFAD/p75NTR ko mice (2mo & 4mo - n = 5 for each genotype). Data are presented as mean ± SEM. 2way ANOVA** p < 0,005, * p < 0,05. BrdU, 5-bromo-2′-deoxyuridine; Sox2, SRY-Box Transcription Factor 2; Dcx, doublecortin

Article Snippet: Wild-type (WT), p75NTR ko, p75 fl/fl NestinCre, 5xFAD and 5xFAD/p75NTR ko male and female mice of different ages (2, 4 and 6-months old) were used. p75NTR ko mice (Ngfrtm1Jae ExonIII) and 5xFAD heterozygous mice (#002213 and #034848-JAX, respectively) were maintained on a C57BL/6 background.

Techniques: Injection

Journal: Alzheimer's Research & Therapy

Article Title: p75 neurotrophin receptor shapes the dynamics of adult hippocampal neurogenesis in Alzheimer’s disease

doi: 10.1186/s13195-026-01989-7

Figure Lengend Snippet: p75NTR expression and function on human iPSCs - derived NSCs. A Representative blots ( n = 3) determined via Western Blot showing the expression of p75NTR, RIP2 and TRAF6 proteins in lysates of two iPSCs-derived NSCs lines (841, 856). Co-IP showing the interaction of p75NTR with TRAF6 protein. HEK293T cells, mNSCs p7 (mouse NSCs from postnatal day 7), PC12 (pheochromocytoma of the rat adrenal medulla), HEK293T cells transiently transfected with p75NTR plasmid and NIH3T3 cells expressing TrkB receptor were used like control samples. kDa, molecular weight; M, Marker for molecular weights, p75NTR (70–75 KDa), actin (42 KDa), TRAF6 (60KDa), RIP2 (61KDa). B Dead cells (green) upon inhibition of p75NTR and/or treatment with aβ oligomers (50 μm scale bar). C Graph showing the percentage of dead cells (Celltox labeled cells/total number of HOECHST + cells) n = 3 from different NSCs lines (841, 856) (one way ANOVA ** p < 0,05, *** p < 0,005, ns, no significant)

Article Snippet: Wild-type (WT), p75NTR ko, p75 fl/fl NestinCre, 5xFAD and 5xFAD/p75NTR ko male and female mice of different ages (2, 4 and 6-months old) were used. p75NTR ko mice (Ngfrtm1Jae ExonIII) and 5xFAD heterozygous mice (#002213 and #034848-JAX, respectively) were maintained on a C57BL/6 background.

Techniques: Expressing, Derivative Assay, Western Blot, Co-Immunoprecipitation Assay, Transfection, Plasmid Preparation, Control, Molecular Weight, Marker, Inhibition, CellTox Assay, Labeling

Journal: Alzheimer's Research & Therapy

Article Title: p75 neurotrophin receptor shapes the dynamics of adult hippocampal neurogenesis in Alzheimer’s disease

doi: 10.1186/s13195-026-01989-7

Figure Lengend Snippet: Functional analysis of differential gene expression driven by the p75NTR ko genotype. A Volcano plot showing the number of Up (9) and Down (33) regulated genes (p adj < 0.05), pointing out the differential expression of Ngfr. Differential expression was determined using a Generalized Linear Model (EdgeR) identifying the main effect of the p75NTR ko genotype (p75NTR ko and 5xFAD/p75NTR ko mice, relative to WT and 5xFAD mice), adjusting for disease status and gender. B Bubble plot showing selected gene ontology (GO) and pathways significantly enriched by the complete list of significant DEGs (both upregulated and downregulated genes) identified in ( A ). Analysis was performed using Metascape (https://metascape.org). The x axis represents the gene ratio. Size of the bubble represents the numbers of DEGs found in each GO list. Coloration from yellow to black represents the – Log10_P value and depicts low (yellow) to high (black) enrichment scores (p adj < 0.05). C Heatmap showing the expression profile of all differentially expressed genes (p adj < 0.05) between samples with (5xFAD/p75NTR ko - green, p75NTR ko - blue) and without (5xFAD - yellow, WT - purple) the p75NTR receptor knocked out. Scaling was performed across genes using z-scores, to highlight relative expression differences. Fold changes are represented using the log₂ fold change (log₂FC) scale to reflect differential expression between conditions

Article Snippet: Wild-type (WT), p75NTR ko, p75 fl/fl NestinCre, 5xFAD and 5xFAD/p75NTR ko male and female mice of different ages (2, 4 and 6-months old) were used. p75NTR ko mice (Ngfrtm1Jae ExonIII) and 5xFAD heterozygous mice (#002213 and #034848-JAX, respectively) were maintained on a C57BL/6 background.

Techniques: Functional Assay, Gene Expression, Quantitative Proteomics, Expressing

Journal: Alzheimer's Research & Therapy

Article Title: p75 neurotrophin receptor shapes the dynamics of adult hippocampal neurogenesis in Alzheimer’s disease

doi: 10.1186/s13195-026-01989-7

Figure Lengend Snippet: The effects of p75NTR deficiency on the proliferation and differentiation of NSCs, under Alzheimer’s Disease. A Coronal sections, of the hippocampal DG from 2 months old 5xFAD/p75NTR ko mouse injected with BrdU for 5 days. Sections were co-immunostained for BrdU (red) and Sox2 (green). Scale bar, 100 μm ( B ) Quantification of BrdU + and Sox2 + cells in injected mice. (2mo & 4mo - n = 5 for each genotype). Data are presented as mean ± SEM. 2way ANOVA**** p < 0,0001, *** p < 0,001, p**< 0,005. C Coronal sections, of the hippocampal DG from 2 months old WT, p75NTR ko, 5xFAD and 5xFAD/p75NTR ko mice. Image depicts Dcx (red) immunostained immature neurons. Scale bar, 100 μm. D Quantification of Dcx + cells in WT, p75NTR ko, 5xFAD, and 5xFAD/p75NTR ko mice (2mo & 4mo - n = 5 for each genotype). Data are presented as mean ± SEM. 2way ANOVA** p < 0,005, * p < 0,05. BrdU, 5-bromo-2′-deoxyuridine; Sox2, SRY-Box Transcription Factor 2; Dcx, doublecortin

Article Snippet: Wild-type (WT), p75NTR ko, p75 fl/fl NestinCre, 5xFAD and 5xFAD/p75NTR ko male and female mice of different ages (2, 4 and 6-months old) were used. p75NTR ko mice (Ngfrtm1Jae ExonIII) and 5xFAD heterozygous mice (#002213 and #034848-JAX, respectively) were maintained on a C57BL/6 background.

Techniques: Injection